SUPPORT

Technical Support and Resources

Explore the latest support resources and FAQs for SimpleCell 3′ Gene Expression kits or reach out to the support team for more information about your project.

SimpleCell Knowledge Base

Access SimpleCell 3′ Gene Expression site prep, workflow, and troubleshooting documentation on the CS Genetics Knowledge Base.

SimpleCell Frequently Asked Questions

What is the SimpleCell 3' Gene Expression Kit used for?

The SimpleCell 3′ Gene Expression Kit is designed to quantify gene expression at the 3′ end of mRNA transcripts. It provides a cost-effective, streamlined solution for transcriptome analysis, making it ideal for high-throughput studies such as population-scale profiling, drug-screening, and translational discovery

The kit is compatible with a wide variety of tissues and cell types including cultured cells, primary cells, organoids, and dissociated solid tissues.

Typical input requirements range from 5,000-8,500 cells, though as little as 1,000 cells may be used for each sample.  In some cases, higher cell inputs can be used to increase output.  For questions, please refer to the Cell Input Requirements section of our support page. 

Yes, the SimpleCell kit is optimized for use with dissociated organoids. Please refer to the Cell Input Requirements section of our support page for input recommendations. 

The library prep workflow produces Illumina-compatible sequencing libraries. It can be used with common Illumina instruments including NovaSeq, NextSeq, and MiSeq platforms. 

Other sequencing platforms may be used (Element AVITI and Oxford Nanopore Technologies).  Please refer to sequencing provider websites for recommendations on converting libraries. 

30,000 reads/cell is the recommended sequencing depth for SimpleCell. 

Library preparation can typically be completed in under 8 hours. 

Sample multiplexing is achieved by using unique dual indexed sequencing adapters. The number of samples that you can multiplex into a single sequencing pool is determined by your desired sequencing depth and the number of UDIs to which you have access. We can supply up to 96 UDIs in our Accessory Starter Kits or you can purchase them from your supplier of choice. For more information or to multiplex more samples, please contact Technical Support

Yes, CS Genetics provides a streamlined, open-source pipeline to process your sequencing outputs. It includes quality control, alignment, deduplication, and a QC report. Compatibility with open-source tools like STAR and Salmon is also supported. You can access the pipeline here: CS Genetics scRNA-seq Pipeline on GitHub 

The kit generates 3′ reads for single-cell analysis. The output will be a count matrix file which is compatible with ScanPy or Seurat for downstream analysis. 

You can reach our technical support team via support@csgenetics.com. To reorder the kit or request a quote, visit our Products page or contact your CS Genetics representative. 

Contact Support

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