PRODUCT

Discover SimpleCell 3’
Gene Expression

SimpleCell combines flexibility, speed, and cost-efficiency for applications ranging from low-volume discovery to population-scale programs.

Redefining Single Cell Library Preparation with Simplicity, Speed, and Unprecedented Scalability

MODULAR THROUGHPUT CAPACITY
PLUG-AND-PLAY AUTOMATION
INSTRUMENT-FREE, ONE TUBE CHEMISTRY
ULTRA-LOW INPUT REQUIREMENTS
WORKFLOW

Single Day Cells-to-Sequencer Workflow

The SimpleCell workflow requires no expensive hardware, complex indexing schemes, or emulsions and takes place entirely within standard laboratory plasticware, at any scale – single tube, 8 -strip, 96-well plates, and beyond.  With multiple fixation-free pause points, cells can be conveniently stored at -80°C or frozen for processing up to 2 months later.

Total Time: 8 h
90 min
Cell Priming & CPair Reaction
70 min
STOP
Optional Pause Point
70 min
Single Cell Barcoding
60 min
3 h
cDNA & Second Strand Synthesis
45 min
90 min
PCR & Enrichment
50 min
STOP
50 min
Indexing Cleanup
25 min
Hands-On Time: 4 h 10 min
KIT AVAILABILITY

Enabling Labs with Cost-Efficient, On-Demand Scalability

SimpleCell’s 8, 16, and 96 sample kits allow labs to choose the exact scale needed for a study — from small pilot experiments to high-throughput screens — without committing to large batch sizes or pooled processing.

Part NumberDescriptionAdditional Information
1000393Simple 3′ Gene Expression 8 SamplesIncludes two boxes of reagents for preparing 8 libraries. Purchase index adapters and required plastics from your supplier or as part of the Accessory Starter Kit 8 Samples.
1000396Accessory Starter Kit 8 SamplesIncludes two boxes of accessory components required for the full assay. Box 1 contains index adapters I1 to I8, and box 2 contains v-bottom and flat-bottom plastic plates.
Part NumberDescriptionAdditional Information
1000394Simple 3′ Gene Expression 16 SamplesIncludes two boxes of reagents for preparing 16 libraries. Purchase index adapters and required plastics from your supplier or as part of the Accessory Starter Kit 8 Samples.
1000406Accessory Starter Kit 16 SamplesIncludes three boxes of accessory components required for the full assay. Box 1 contains index adapters I1 to I8, box 2 contains adapters I9 to I16 and box 3 contains v-bottom and flat-bottom plastic plates.
Part NumberDescriptionAdditional Information
1000517Simple 3′ Gene Expression 96 SamplesIncludes two boxes of reagents for preparing 96 libraries. Purchase index adapters and required plastics from your supplier or as part of the Accessory Starter Kit 8 Samples.
1000518Accessory Starter Kit 96 SamplesIncludes two boxes of accessory components required for the full assay. Box 1 contains index adapters I1 to I96 and box 2 contains v-bottom and flat-bottom plastic plates.
IMPACT

Faster Insights at a Fraction of the Price

SimpleCell technology has been designed with the user in mind, enabling up to 12x more samples to be run per week than the leading single cell library prep technology, at a fraction of the cost.

CUSTOMER SPOTLIGHT

Validated by Industry Leading Labs & Researchers

PERFORMANCE

Powerful Biological Insights with a Simple & Scalable Workflow

Comparison of PBMCs Between Technologies

An early-access user was interested in improving efficiency and simplifying workflows using the CS Genetics SimpleCell™ Assay. Viably frozen PBMCs were processed with the SimpleCell™ 3′ Gene Expression Assay and with an alternative 3′ Gene Expression technology. Data from the two technologies were analysed separately and revealed the same cell type populations with relatively similar abundance. The data were then split by donor and integrated computationally. There was an excellent overlap between the data sets from the two technologies regardless of donor.    

96 Frozen Whole Blood Samples

An early-access user was interested in a simplified high throughput single cell assay that works with frozen whole-blood samples to enable population scale single cell analysis. The CS Genetics SimpleCell™ Assay was used to process 96 frozen whole-blood samples in two days with the aid of a 96-channel semi-automated pipettor, thus showcasing the scalability of the technology. Gene expression quantification enabled high-resolution annotation of cell types within the samples. 

iPSCs Differentiation

An early-access user differentiated Human iPSCs to cortical neurons using an iNeuron protocol. Samples were collected in triplicate at 5 time points with a total of 15 processed for single cell RNA sequencing. Analysis of these combined datasets revealed distinct developmental states of the iPSCs during differentiation. Unsupervised pseudotime ordering revealed 4 key developmental stages, beginning with a formative stem-cell stage at D0, followed by a progenitor stage at D4. These were followed by more developmentally heterogenous stages up to D14, with cells existing in several pseudotime states (also potentially representing different developing neural types) while by D30 most cells reside in a mature state.

CAR-T Cell Screening Comparison to an Alternative Single Cell Technology

Single cell sequencing can be used to find phenotypes of cells beyond what is possible with flow cytometry but, unfortunately, the cost with single cell technologies currently on the market can be prohibitively expensive for large scale applications like CAR-T donor screening. The CS Genetics SimpleCell™ Assay offers an easy, scalable and cost-effective solution for these applications with comparable performance to current technologies.  An early-access user compared SimpleCell™ with another single cell technology and demonstrated that both datasets revealed comparable biological insights.

Quantification of a therapeutically-relevant gene-seq enrichment analysis (GSEA) signature demonstrated excellent correlation between the two technologies. The CS Genetics SimpleCell™ Assay showed similar quality, phenotypes and patterns in the transcriptional profiles.  

Get Started with SimpleCell

Please provide your information and a brief description of your project and a member of the CS Genetics team will be in contact to set up a meeting.

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